Spatial processing using 10X SpaceRanger
Generated by: George Chlipala
Report date: February 27, 2026
Overview
When you publish manuscripts based on data generated at our facility, we would greatly appreciate an acknowledgement of our efforts. Please cite our facility as follows (for example):
Basic processing of the raw data were performed by the University of Illinois at Chicago Research Informatics Core (UICRIC).
We adhere to a general policy for acknowledgements and authorship as established by the Association for Biomolecular Resource Facilities (ABRF) , and we support the following statement from the ABRF.
The existence of core facilities depends in part on proper acknowledgment in publications. This is an important metric of the value of most core facilities. Proper acknowledgment of core facilities enables them to obtain financial and other support so that they may continue to provide their essential services in the best ways possible. It also helps core personnel to advance in their careers, adding to the overall health of the core facility.
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| File | Description | Type |
|---|---|---|
| Visium_HD_3prime_Mouse_Kidney.zip | SpaceRanger output for Visium_HD_3prime_Mouse_Kidney | result |
| Sample | OriginalID |
|---|---|
| Visium_HD_3prime_Mouse_Kidney | Visium_HD_3prime_Mouse_Kidney |
- Method: Processing of 10X Genomics Visium data with SpaceRangerhttps://www.10xgenomics.com/support/software/space-ranger/latest
-
Whole transcriptome is mapped to tissue on a microscope image. - Reference sequence database : mm10
-
CellRanger reference genome for mouse mm10, November 19, 2020.
Figure 1 . Gene expression (GEX) sample summary
Table 1 . Data processing summary statistics of gene expression samples
| Sample | Reads Mapped Confidently to Genome | Reads Mapped Confidently to Transcriptome | Total Genes Detected | Valid Barcodes | Valid UMI Sequences | Number of Reads | Number of Squares Under Tissue 2 µm | Number of Bins Under Tissue 8 µm | Number of Bins Under Tissue 16 µm | Number of Cells | Median Genes per Cell | Median UMIs per Cell | Median Cell Area (μm²) | Median Nucleus Area (μm²) | Mean UMIs Under Tissue per Square 2 µm | Mean UMIs Under Tissue per Bin 8 µm | Mean UMIs Under Tissue per Bin 16 µm | Mean Genes Under Tissue per Square 2 µm | Mean Genes Under Tissue per Bin 8 µm | Mean Genes Under Tissue per Bin 16 µm |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Visium_HD_3prime_Mouse_Kidney | 0.8216008740353596 | 0.7399878736144156 | 20957 | 0.8791618810312359 | 0.9947524507028236 | 520565667 | 6753403 | 423482 | 106302 | 277215 | 238 | 310 | 84 | 24 | 20.749393602011363 | 331.06829570088036 | 1319.5468570676042 | 18.52003678737899 | 236.8115575160226 | 759.6465635641824 |