Denovo genome assembly using short read data
Generated by: George Edward Chlipala
Report date: April 17, 2024
Overview
When you publish manuscripts based on data generated at our facility, we would greatly appreciate an acknowledgement of our efforts. Please cite our facility as follows (for example):
Basic processing of the raw data were performed by the University of Illinois at Chicago Research Informatics Core (UICRIC).
We adhere to a general policy for acknowledgements and authorship as established by the Association for Biomolecular Resource Facilities (ABRF) , and we support the following statement from the ABRF.
The existence of core facilities depends in part on proper acknowledgment in publications. This is an important metric of the value of most core facilities. Proper acknowledgment of core facilities enables them to obtain financial and other support so that they may continue to provide their essential services in the best ways possible. It also helps core personnel to advance in their careers, adding to the overall health of the core facility.
Please contact us for assistance in drafting manuscripts.
Output Files
File | Description | Type |
---|---|---|
example.gene-AA.fa | Protein sequences of predicted ORFs for example | result |
example.gene-NT.fa | Nucleotide sequences of predicted ORFs for example | result |
example.annotation.txt | Annotations of predicted ORFs for example | result |
example.gff | Details of predicted ORFs for example in GFF format | result |
example.gbk | Annotated sequences of contigs for example | result |
example.raw_assembly.zip | ZIP compressed FASTA file of all contigs and scaffolds for example | result |
example.assembly.zip | ZIP compressed FASTA file of filtered contigs and scaffolds for example | result |
Name | OriginalID |
---|---|
example | example |
Details
- Method: cutadapt
-
Sequencing trimming using cutadapt - --report = minimal
- -m = 70
- --trim-n
- --max-n = 1
- -q = 10
- -b = file:/mnt/store3/clustcrilab/reference/other/primers.fa
- Method: Quality trimming
-
Quality trimming based on quality threshold and length parameters. - p = 10
- min length = 70
- Method: Adapter trimming
-
Adapter/primer sequences were trimmed from the reads. - adapter = TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
- adapter = GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
- adapter = CAAGCAGAAGACGGCATACGAGAT
- adapter = AATGATACGGCGACCACCGAGATCTACAC
- adapter = AATGATACGGCGACCACCGAGATCTACACGTAAGGAGTCGTCGGCAGCGTC
- adapter = CAGTTACGTATTGC
- adapter = CGTTCTGTTTATGTTTCTTG
- Method: Abiguous nucleotide trimming
-
Ambiguous nucleotides (N) were trimmed from the ends and reads with internal ambiguous nucleotides were discarded. - max-n = 1
Figure 1. Trimming results
Table 1. Trimming statistics Download table data
Sample | Trim passed | Ambig dropped | Trim filter dropped | Trim length dropped | Percent passed | Mean Length | Mean Trimmed Length | Quality trimmed bp |
---|---|---|---|---|---|---|---|---|
example | 9504850 | 708 | 0 | 138755 | 98.55% | 292.6 | NA | 258766 |
Details
- Method: SPAdes assembler
-
SPAdes - St. Petersburg genome assembler is an assembly toolkit containing various denovo genome assembly pipelines. - -k = 27,47,67,87,107,127
Figure 1. Coverage plot for example
Table 1. Assembly summary for all samples Download table data
Sample | Count | TotalLength | Longest | N50 | N75 | N90 | L50 | L75 | L90 |
---|---|---|---|---|---|---|---|---|---|
example | 119 | 1789508 | 182623 | 98522 | 42066 | 18670 | 7 | 14 | 24 |
Table 2. Scaffold summary for all samples Download table data
Sample | Count | TotalLength | Longest | N50 | N75 | N90 | L50 | L75 | L90 |
---|---|---|---|---|---|---|---|---|---|
example | 119 | 1789508 | 182623 | 98522 | 42066 | 18670 | 7 | 14 | 24 |
Table 3. Basic contig statistics for example Download table data
#ID | length | GC | coverage | C50 | C75 | C90 | S_2mer | S_3mer | S_4mer |
---|---|---|---|---|---|---|---|---|---|
NODE_1 | 182623 | 33.467 | 684.59 | 646.00 | 371.00 | 181.00 | 0.956 | 0.954 | 0.952 |
NODE_2 | 178351 | 34.587 | 1253.42 | 1237.00 | 765.00 | 442.00 | 0.959 | 0.956 | 0.953 |
NODE_3 | 168600 | 34.098 | 1914.56 | 1841.00 | 1159.00 | 613.00 | 0.958 | 0.955 | 0.953 |
NODE_4 | 108610 | 33.987 | 1071.17 | 1032.00 | 588.00 | 305.00 | 0.955 | 0.953 | 0.950 |
NODE_5 | 106404 | 34.917 | 2697.86 | 2140.00 | 1271.00 | 705.00 | 0.963 | 0.961 | 0.958 |
NODE_6 | 104422 | 34.860 | 1578.88 | 1564.00 | 872.00 | 468.00 | 0.961 | 0.958 | 0.955 |
NODE_7 | 98522 | 34.342 | 1482.21 | 1444.00 | 816.00 | 445.00 | 0.958 | 0.956 | 0.954 |
NODE_8 | 92742 | 35.171 | 1287.58 | 1280.00 | 807.00 | 467.00 | 0.962 | 0.959 | 0.956 |
NODE_9 | 70410 | 34.789 | 1079.93 | 1066.00 | 675.00 | 382.00 | 0.959 | 0.956 | 0.952 |
NODE_10 | 57454 | 34.495 | 788.26 | 782.00 | 472.00 | 271.00 | 0.958 | 0.955 | 0.952 |
NODE_11 | 56714 | 33.849 | 1357.29 | 1297.00 | 751.00 | 411.00 | 0.956 | 0.953 | 0.951 |
NODE_12 | 52901 | 34.379 | 1834.21 | 1802.00 | 1116.00 | 639.00 | 0.960 | 0.957 | 0.955 |
NODE_13 | 46487 | 35.145 | 1254.11 | 1212.00 | 662.00 | 357.00 | 0.962 | 0.959 | 0.956 |
NODE_14 | 42066 | 34.522 | 2197.40 | 2195.00 | 1435.00 | 818.00 | 0.960 | 0.958 | 0.955 |
NODE_15 | 35934 | 32.807 | 1004.74 | 907.00 | 367.00 | 171.00 | 0.951 | 0.949 | 0.947 |
NODE_16 | 28356 | 34.240 | 1324.76 | 1299.00 | 733.00 | 394.00 | 0.959 | 0.957 | 0.954 |
NODE_17 | 26026 | 33.832 | 1860.52 | 1819.00 | 1090.00 | 648.00 | 0.957 | 0.955 | 0.953 |
NODE_18 | 25832 | 34.821 | 2181.03 | 2042.00 | 1355.00 | 874.00 | 0.961 | 0.958 | 0.956 |
NODE_19 | 24499 | 35.067 | 2000.36 | 2010.00 | 1252.00 | 743.00 | 0.961 | 0.957 | 0.954 |
NODE_20 | 23272 | 34.849 | 1090.49 | 1014.00 | 569.00 | 340.00 | 0.962 | 0.958 | 0.955 |
NODE_21 | 21956 | 34.305 | 1264.71 | 1263.00 | 801.00 | 463.00 | 0.961 | 0.959 | 0.957 |
NODE_22 | 20259 | 35.283 | 737.14 | 750.00 | 503.00 | 295.00 | 0.962 | 0.959 | 0.956 |
NODE_23 | 19734 | 33.845 | 1386.03 | 1366.00 | 837.00 | 400.00 | 0.954 | 0.951 | 0.948 |
NODE_24 | 18670 | 33.476 | 2175.37 | 1811.00 | 1183.00 | 684.00 | 0.954 | 0.951 | 0.949 |
NODE_25 | 16449 | 38.853 | 2190.79 | 2214.00 | 1434.00 | 801.00 | 0.977 | 0.975 | 0.972 |
NODE_26 | 16232 | 34.709 | 1480.97 | 1486.00 | 702.00 | 321.00 | 0.961 | 0.959 | 0.957 |
NODE_27 | 13532 | 34.599 | 2265.18 | 2319.00 | 1572.00 | 884.00 | 0.957 | 0.952 | 0.948 |
NODE_28 | 12209 | 35.621 | 2700.38 | 2847.00 | 1619.00 | 717.00 | 0.965 | 0.962 | 0.958 |
NODE_29 | 12075 | 34.791 | 1381.33 | 1382.00 | 982.00 | 622.00 | 0.960 | 0.956 | 0.952 |
NODE_30 | 10228 | 34.523 | 2201.04 | 2059.00 | 1390.00 | 884.00 | 0.959 | 0.957 | 0.953 |
NODE_31 | 9310 | 37.787 | 1678.93 | 1347.00 | 840.00 | 434.00 | 0.973 | 0.971 | 0.968 |
NODE_32 | 9224 | 35.755 | 2475.87 | 2501.00 | 1417.00 | 830.00 | 0.966 | 0.964 | 0.959 |
NODE_33 | 8031 | 32.611 | 457.91 | 415.00 | 252.00 | 124.00 | 0.949 | 0.946 | 0.942 |
NODE_34 | 7838 | 34.894 | 1076.54 | 976.00 | 726.00 | 526.00 | 0.960 | 0.957 | 0.954 |
NODE_35 | 7357 | 33.342 | 1204.73 | 1136.00 | 467.00 | 144.00 | 0.953 | 0.949 | 0.945 |
NODE_36 | 6445 | 39.472 | 3057.43 | 2686.00 | 2090.00 | 1546.00 | 0.971 | 0.963 | 0.954 |
NODE_37 | 5447 | 44.924 | 2366.60 | 2344.00 | 2083.00 | 1893.00 | 0.991 | 0.984 | 0.978 |
NODE_38 | 3151 | 48.969 | 17347.93 | 18029.00 | 16366.00 | 15164.00 | 0.982 | 0.980 | 0.975 |
NODE_39 | 2356 | 32.598 | 565.08 | 592.00 | 347.00 | 234.00 | 0.944 | 0.940 | 0.930 |
NODE_40 | 2319 | 43.812 | 3712.25 | 3725.00 | 3417.00 | 2878.00 | 0.971 | 0.927 | 0.871 |
NODE_41 | 2269 | 34.156 | 4999.76 | 5231.00 | 4312.00 | 2569.00 | 0.956 | 0.951 | 0.941 |
NODE_42 | 1969 | 48.400 | 17229.92 | 17797.00 | 15229.00 | 11153.00 | 0.988 | 0.984 | 0.973 |
NODE_43 | 1681 | 41.761 | 20095.20 | 21745.00 | 18287.00 | 10158.00 | 0.978 | 0.967 | 0.951 |
NODE_44 | 1666 | 42.317 | 5514.56 | 5961.00 | 5026.00 | 3017.00 | 0.986 | 0.981 | 0.970 |
NODE_45 | 1461 | 32.512 | 7031.37 | 8487.00 | 1585.00 | 1003.00 | 0.949 | 0.942 | 0.926 |
NODE_46 | 1306 | 31.700 | 740.65 | 801.00 | 362.00 | 162.00 | 0.946 | 0.941 | 0.926 |
NODE_47 | 1301 | 41.583 | 4393.83 | 4412.00 | 4145.00 | 3464.00 | 0.966 | 0.946 | 0.921 |
NODE_48 | 1194 | 39.112 | 3574.48 | 4156.00 | 3428.00 | 716.00 | 0.977 | 0.969 | 0.953 |
NODE_49 | 1189 | 37.426 | 7669.21 | 8442.00 | 7107.00 | 2700.00 | 0.972 | 0.966 | 0.949 |
NODE_50 | 848 | 38.797 | 6.27 | 6.00 | 5.00 | 2.00 | 0.959 | 0.938 | 0.891 |
NODE_51 | 844 | 39.455 | 6.28 | 6.00 | 4.00 | 1.00 | 0.943 | 0.931 | 0.908 |
NODE_52 | 657 | 39.422 | 5.42 | 5.00 | 3.00 | 2.00 | 0.938 | 0.922 | 0.892 |
NODE_53 | 562 | 35.053 | 4135.60 | 5035.00 | 2381.00 | 844.00 | 0.955 | 0.941 | 0.904 |
NODE_54 | 498 | 45.382 | 6505.51 | 7752.00 | 4339.00 | 2186.00 | 0.969 | 0.953 | 0.911 |
NODE_55 | 456 | 48.465 | 17.98 | 8.00 | 2.00 | 1.00 | 0.984 | 0.964 | 0.919 |
NODE_56 | 413 | 38.499 | 1.38 | 1.00 | 1.00 | 1.00 | 0.930 | 0.913 | 0.871 |
NODE_57 | 409 | 40.587 | 3.40 | 4.00 | 2.00 | 1.00 | 0.965 | 0.939 | 0.886 |
NODE_58 | 407 | 38.821 | 1.72 | 2.00 | 1.00 | 1.00 | 0.937 | 0.921 | 0.884 |
NODE_59 | 392 | 32.398 | 1021.08 | 1188.00 | 743.00 | 333.00 | 0.947 | 0.931 | 0.884 |
NODE_60 | 381 | 39.108 | 3.68 | 2.00 | 1.00 | 1.00 | 0.967 | 0.946 | 0.898 |
NODE_61 | 379 | 41.161 | 66.46 | 20.00 | 7.00 | 2.00 | 0.980 | 0.964 | 0.913 |
NODE_62 | 376 | 37.500 | 28.62 | 26.00 | 16.00 | 3.00 | 0.968 | 0.936 | 0.880 |
NODE_63 | 368 | 34.511 | 29.71 | 15.00 | 8.00 | 0.00 | 0.952 | 0.923 | 0.874 |
NODE_64 | 354 | 50.847 | 85.60 | 21.00 | 6.00 | 5.00 | 0.983 | 0.970 | 0.917 |
NODE_65 | 353 | 37.110 | 753.38 | 876.00 | 469.00 | 379.00 | 0.955 | 0.930 | 0.870 |
NODE_66 | 337 | 42.136 | 1.34 | 1.00 | 1.00 | 1.00 | 0.966 | 0.951 | 0.892 |
NODE_67 | 336 | 42.857 | 6.22 | 6.00 | 2.00 | 0.00 | 0.979 | 0.939 | 0.877 |
NODE_68 | 327 | 54.434 | 470.35 | 210.00 | 10.00 | 4.00 | 0.990 | 0.973 | 0.906 |
NODE_69 | 326 | 46.933 | 44.61 | 49.00 | 12.00 | 7.00 | 0.969 | 0.954 | 0.902 |
NODE_70 | 326 | 53.067 | 12.85 | 16.00 | 3.00 | 1.00 | 0.990 | 0.971 | 0.914 |
NODE_71 | 325 | 38.462 | 1.81 | 2.00 | 1.00 | 1.00 | 0.933 | 0.919 | 0.875 |
NODE_72 | 315 | 41.270 | 688.01 | 713.00 | 624.00 | 335.00 | 0.954 | 0.927 | 0.871 |
NODE_73 | 315 | 36.508 | 1.90 | 2.00 | 1.00 | 1.00 | 0.897 | 0.879 | 0.828 |
NODE_74 | 313 | 40.575 | 2966.10 | 3041.00 | 1420.00 | 679.00 | 0.954 | 0.929 | 0.875 |
NODE_75 | 311 | 42.444 | 519.19 | 424.00 | 121.00 | 5.00 | 0.968 | 0.931 | 0.865 |
NODE_76 | 307 | 41.694 | 14473.75 | 14135.00 | 5771.00 | 2266.00 | 0.959 | 0.919 | 0.847 |
NODE_77 | 306 | 50.327 | 19.62 | 17.00 | 3.00 | 2.00 | 0.982 | 0.963 | 0.901 |
NODE_78 | 305 | 44.262 | 7.70 | 7.00 | 1.00 | 1.00 | 0.972 | 0.954 | 0.898 |
NODE_79 | 304 | 46.711 | 10.71 | 7.00 | 1.00 | 0.00 | 0.970 | 0.942 | 0.883 |
NODE_80 | 301 | 38.870 | 7.26 | 8.00 | 7.00 | 2.00 | 0.959 | 0.931 | 0.867 |
NODE_81 | 298 | 33.893 | 25.34 | 18.00 | 13.00 | 5.00 | 0.936 | 0.914 | 0.855 |
NODE_82 | 298 | 35.235 | 9.32 | 6.00 | 2.00 | 1.00 | 0.960 | 0.926 | 0.865 |
NODE_83 | 297 | 49.158 | 1.02 | 1.00 | 1.00 | 1.00 | 0.971 | 0.952 | 0.887 |
NODE_84 | 294 | 55.442 | 1.52 | 1.00 | 1.00 | 1.00 | 0.985 | 0.968 | 0.909 |
NODE_85 | 294 | 46.939 | 1.54 | 2.00 | 1.00 | 1.00 | 0.977 | 0.950 | 0.885 |
NODE_86 | 293 | 43.345 | 7943.56 | 8959.00 | 3320.00 | 1435.00 | 0.967 | 0.926 | 0.859 |
NODE_87 | 293 | 49.829 | 14.19 | 10.00 | 7.00 | 4.00 | 0.986 | 0.971 | 0.907 |
NODE_88 | 293 | 46.758 | 8.60 | 3.00 | 1.00 | 0.00 | 0.983 | 0.966 | 0.903 |
NODE_89 | 292 | 45.548 | 2.07 | 2.00 | 2.00 | 2.00 | 0.988 | 0.967 | 0.895 |
NODE_90 | 292 | 46.918 | 7.93 | 4.00 | 1.00 | 1.00 | 0.980 | 0.956 | 0.885 |
NODE_91 | 291 | 34.021 | 5.95 | 6.00 | 1.00 | 0.00 | 0.950 | 0.901 | 0.837 |
NODE_92 | 290 | 41.379 | 6.68 | 5.00 | 2.00 | 1.00 | 0.955 | 0.913 | 0.851 |
NODE_93 | 290 | 41.724 | 68.56 | 51.00 | 16.00 | 12.00 | 0.960 | 0.928 | 0.864 |
NODE_94 | 287 | 44.251 | 8.97 | 11.00 | 3.00 | 1.00 | 0.966 | 0.931 | 0.864 |
NODE_95 | 287 | 41.812 | 78.16 | 14.00 | 12.00 | 7.00 | 0.972 | 0.941 | 0.883 |
NODE_96 | 287 | 31.707 | 2.38 | 2.00 | 2.00 | 2.00 | 0.888 | 0.868 | 0.816 |
NODE_97 | 287 | 37.282 | 3.01 | 3.00 | 2.00 | 1.00 | 0.924 | 0.904 | 0.853 |
NODE_98 | 286 | 39.510 | 2140.09 | 2266.00 | 1187.00 | 710.00 | 0.964 | 0.939 | 0.866 |
NODE_99 | 286 | 44.406 | 37.02 | 38.00 | 15.00 | 9.00 | 0.970 | 0.927 | 0.860 |
NODE_100 | 283 | 51.943 | 2.13 | 2.00 | 2.00 | 2.00 | 0.955 | 0.921 | 0.855 |
NODE_101 | 281 | 56.228 | 19.95 | 23.00 | 11.00 | 1.00 | 0.985 | 0.963 | 0.903 |
NODE_102 | 281 | 31.317 | 9.78 | 8.00 | 7.00 | 5.00 | 0.919 | 0.896 | 0.841 |
NODE_103 | 281 | 30.961 | 2.14 | 2.00 | 1.00 | 1.00 | 0.866 | 0.796 | 0.728 |
NODE_104 | 281 | 43.060 | 75.62 | 33.00 | 23.00 | 13.00 | 0.961 | 0.928 | 0.860 |
NODE_105 | 280 | 45.000 | 1.07 | 1.00 | 1.00 | 1.00 | 0.976 | 0.955 | 0.885 |
NODE_106 | 271 | 44.649 | 10100.45 | 10856.00 | 5730.00 | 2848.00 | 0.962 | 0.922 | 0.855 |
NODE_107 | 267 | 42.697 | 3611.71 | 3694.00 | 2046.00 | 1091.00 | 0.938 | 0.912 | 0.850 |
NODE_108 | 255 | 40.784 | 2437.40 | 2426.00 | 1601.00 | 776.00 | 0.950 | 0.916 | 0.853 |
NODE_109 | 223 | 43.498 | 17778.34 | 19280.00 | 8271.00 | 4438.00 | 0.959 | 0.915 | 0.841 |
NODE_110 | 216 | 41.667 | 1358.76 | 1434.00 | 817.00 | 425.00 | 0.940 | 0.899 | 0.829 |
NODE_111 | 211 | 41.232 | 1140.87 | 1287.00 | 630.00 | 313.00 | 0.954 | 0.916 | 0.846 |
NODE_112 | 197 | 42.640 | 15638.01 | 16852.00 | 8738.00 | 4369.00 | 0.966 | 0.919 | 0.835 |
NODE_113 | 192 | 44.271 | 17750.68 | 21036.00 | 11652.00 | 6118.00 | 0.951 | 0.905 | 0.826 |
NODE_114 | 185 | 42.162 | 1611.31 | 1935.00 | 1052.00 | 440.00 | 0.958 | 0.922 | 0.836 |
NODE_115 | 171 | 40.351 | 278.75 | 313.00 | 173.00 | 65.00 | 0.925 | 0.869 | 0.790 |
NODE_116 | 166 | 43.373 | 2173.89 | 2479.00 | 1976.00 | 1009.00 | 0.967 | 0.913 | 0.817 |
NODE_117 | 142 | 40.845 | 121.27 | 125.00 | 115.00 | 88.00 | 0.966 | 0.879 | 0.782 |
NODE_118 | 128 | 100.000 | 3778.66 | 3726.00 | 3475.00 | 3062.00 | 0.000 | 0.000 | 0.000 |
NODE_119 | 128 | 30.469 | 19.09 | 20.00 | 18.00 | 16.00 | 0.920 | 0.870 | 0.781 |
Table 4. Basic scaffold statistics for example Download table data
#ID | length | GC | coverage | C50 | C75 | C90 | S_2mer | S_3mer | S_4mer |
---|---|---|---|---|---|---|---|---|---|
NODE_1 | 182623 | 33.467 | 684.59 | 646.00 | 371.00 | 181.00 | 0.956 | 0.954 | 0.952 |
NODE_2 | 178351 | 34.587 | 1253.42 | 1237.00 | 765.00 | 442.00 | 0.959 | 0.956 | 0.953 |
NODE_3 | 168600 | 34.098 | 1914.56 | 1841.00 | 1159.00 | 613.00 | 0.958 | 0.955 | 0.953 |
NODE_4 | 133226 | 35.728 | 2681.58 | 2214.00 | 1341.00 | 739.00 | 0.972 | 0.967 | 0.963 |
NODE_5 | 108610 | 33.987 | 1071.17 | 1032.00 | 588.00 | 305.00 | 0.955 | 0.953 | 0.950 |
NODE_6 | 104422 | 34.860 | 1578.88 | 1564.00 | 872.00 | 468.00 | 0.961 | 0.958 | 0.955 |
NODE_7 | 98522 | 34.342 | 1482.21 | 1444.00 | 816.00 | 445.00 | 0.958 | 0.956 | 0.954 |
NODE_8 | 92742 | 35.171 | 1287.58 | 1280.00 | 807.00 | 467.00 | 0.962 | 0.959 | 0.956 |
NODE_9 | 70410 | 34.789 | 1079.93 | 1066.00 | 675.00 | 382.00 | 0.959 | 0.956 | 0.952 |
NODE_10 | 65210 | 34.559 | 1995.08 | 1893.00 | 1164.00 | 644.00 | 0.964 | 0.960 | 0.957 |
NODE_11 | 57454 | 34.495 | 788.27 | 782.00 | 472.00 | 271.00 | 0.958 | 0.955 | 0.952 |
NODE_12 | 56714 | 33.849 | 1357.29 | 1297.00 | 751.00 | 411.00 | 0.956 | 0.953 | 0.951 |
NODE_13 | 46487 | 35.145 | 1254.12 | 1212.00 | 662.00 | 357.00 | 0.962 | 0.959 | 0.956 |
NODE_14 | 42066 | 34.522 | 2197.39 | 2195.00 | 1435.00 | 818.00 | 0.960 | 0.958 | 0.955 |
NODE_15 | 35934 | 32.807 | 1004.74 | 907.00 | 367.00 | 171.00 | 0.951 | 0.949 | 0.947 |
NODE_16 | 28356 | 34.240 | 1324.76 | 1299.00 | 733.00 | 394.00 | 0.959 | 0.957 | 0.954 |
NODE_17 | 26026 | 33.832 | 1860.52 | 1819.00 | 1090.00 | 648.00 | 0.957 | 0.955 | 0.953 |
NODE_18 | 25832 | 34.821 | 2181.03 | 2042.00 | 1355.00 | 874.00 | 0.961 | 0.958 | 0.956 |
NODE_19 | 24499 | 35.067 | 2000.36 | 2010.00 | 1252.00 | 743.00 | 0.961 | 0.957 | 0.954 |
NODE_20 | 23272 | 34.849 | 1090.49 | 1014.00 | 569.00 | 340.00 | 0.962 | 0.958 | 0.955 |
NODE_21 | 21956 | 34.305 | 1264.71 | 1263.00 | 801.00 | 463.00 | 0.961 | 0.959 | 0.957 |
NODE_22 | 20259 | 35.283 | 737.13 | 750.00 | 503.00 | 295.00 | 0.962 | 0.959 | 0.956 |
NODE_23 | 19734 | 33.845 | 1386.08 | 1366.00 | 837.00 | 400.00 | 0.954 | 0.951 | 0.948 |
NODE_24 | 18670 | 33.476 | 2175.37 | 1811.00 | 1183.00 | 684.00 | 0.954 | 0.951 | 0.949 |
NODE_25 | 16232 | 34.709 | 1480.97 | 1486.00 | 702.00 | 321.00 | 0.961 | 0.959 | 0.957 |
NODE_26 | 13532 | 34.599 | 2265.18 | 2319.00 | 1572.00 | 884.00 | 0.957 | 0.952 | 0.948 |
NODE_27 | 12075 | 34.791 | 1381.32 | 1382.00 | 982.00 | 622.00 | 0.960 | 0.956 | 0.952 |
NODE_28 | 10228 | 34.523 | 2201.04 | 2059.00 | 1390.00 | 884.00 | 0.959 | 0.957 | 0.953 |
NODE_29 | 9310 | 37.787 | 1678.93 | 1347.00 | 840.00 | 434.00 | 0.973 | 0.971 | 0.968 |
NODE_30 | 9224 | 35.755 | 2475.87 | 2501.00 | 1417.00 | 830.00 | 0.966 | 0.964 | 0.959 |
NODE_31 | 8031 | 32.611 | 457.91 | 415.00 | 252.00 | 124.00 | 0.949 | 0.946 | 0.942 |
NODE_32 | 7838 | 34.894 | 1076.54 | 976.00 | 726.00 | 526.00 | 0.960 | 0.957 | 0.954 |
NODE_33 | 7357 | 33.342 | 1204.73 | 1136.00 | 467.00 | 144.00 | 0.953 | 0.949 | 0.945 |
NODE_34 | 5447 | 44.924 | 2366.60 | 2344.00 | 2083.00 | 1893.00 | 0.991 | 0.984 | 0.978 |
NODE_35 | 5220 | 47.816 | 16992.86 | 17913.00 | 16053.00 | 11899.00 | 1.001 | 0.988 | 0.978 |
NODE_36 | 2356 | 32.598 | 565.08 | 592.00 | 347.00 | 234.00 | 0.944 | 0.940 | 0.930 |
NODE_37 | 2269 | 34.156 | 4999.76 | 5231.00 | 4312.00 | 2569.00 | 0.956 | 0.951 | 0.941 |
NODE_38 | 1681 | 41.761 | 20094.71 | 21745.00 | 18287.00 | 10150.00 | 0.978 | 0.967 | 0.951 |
NODE_39 | 1666 | 42.317 | 5514.56 | 5961.00 | 5026.00 | 3017.00 | 0.986 | 0.981 | 0.970 |
NODE_40 | 1461 | 32.512 | 7031.37 | 8487.00 | 1585.00 | 1003.00 | 0.949 | 0.942 | 0.926 |
NODE_41 | 1306 | 31.700 | 740.65 | 801.00 | 362.00 | 162.00 | 0.946 | 0.941 | 0.926 |
NODE_42 | 1194 | 39.112 | 3574.48 | 4156.00 | 3428.00 | 716.00 | 0.977 | 0.969 | 0.953 |
NODE_43 | 1189 | 37.426 | 7669.21 | 8442.00 | 7107.00 | 2700.00 | 0.972 | 0.966 | 0.949 |
NODE_44 | 848 | 38.797 | 6.27 | 6.00 | 5.00 | 2.00 | 0.959 | 0.938 | 0.891 |
NODE_45 | 844 | 39.455 | 6.28 | 6.00 | 4.00 | 1.00 | 0.943 | 0.931 | 0.908 |
NODE_46 | 657 | 39.422 | 5.42 | 5.00 | 3.00 | 2.00 | 0.938 | 0.922 | 0.892 |
NODE_47 | 562 | 35.053 | 4135.60 | 5035.00 | 2381.00 | 844.00 | 0.955 | 0.941 | 0.904 |
NODE_48 | 498 | 45.382 | 6439.95 | 7752.00 | 4163.00 | 2011.00 | 0.969 | 0.953 | 0.911 |
NODE_49 | 456 | 48.465 | 17.98 | 8.00 | 2.00 | 1.00 | 0.984 | 0.964 | 0.919 |
NODE_50 | 413 | 38.499 | 1.38 | 1.00 | 1.00 | 1.00 | 0.930 | 0.913 | 0.871 |
NODE_51 | 409 | 40.587 | 3.40 | 4.00 | 2.00 | 1.00 | 0.965 | 0.939 | 0.886 |
NODE_52 | 407 | 38.821 | 1.72 | 2.00 | 1.00 | 1.00 | 0.937 | 0.921 | 0.884 |
NODE_53 | 392 | 32.398 | 1021.08 | 1188.00 | 743.00 | 333.00 | 0.947 | 0.931 | 0.884 |
NODE_54 | 381 | 39.108 | 3.68 | 2.00 | 1.00 | 1.00 | 0.967 | 0.946 | 0.898 |
NODE_55 | 379 | 41.161 | 66.46 | 20.00 | 7.00 | 2.00 | 0.980 | 0.964 | 0.913 |
NODE_56 | 376 | 37.500 | 28.62 | 26.00 | 16.00 | 3.00 | 0.968 | 0.936 | 0.880 |
NODE_57 | 368 | 34.511 | 29.71 | 15.00 | 8.00 | 0.00 | 0.952 | 0.923 | 0.874 |
NODE_58 | 354 | 50.847 | 85.60 | 21.00 | 6.00 | 5.00 | 0.983 | 0.970 | 0.917 |
NODE_59 | 353 | 37.110 | 753.38 | 876.00 | 469.00 | 379.00 | 0.955 | 0.930 | 0.870 |
NODE_60 | 337 | 42.136 | 1.34 | 1.00 | 1.00 | 1.00 | 0.966 | 0.951 | 0.892 |
NODE_61 | 336 | 42.857 | 5.68 | 6.00 | 2.00 | 0.00 | 0.979 | 0.939 | 0.877 |
NODE_62 | 327 | 54.434 | 421.56 | 195.00 | 9.00 | 3.00 | 0.990 | 0.973 | 0.906 |
NODE_63 | 326 | 46.933 | 40.38 | 45.00 | 11.00 | 6.00 | 0.969 | 0.954 | 0.902 |
NODE_64 | 326 | 53.067 | 12.85 | 16.00 | 3.00 | 1.00 | 0.990 | 0.971 | 0.914 |
NODE_65 | 325 | 38.462 | 1.81 | 2.00 | 1.00 | 1.00 | 0.933 | 0.919 | 0.875 |
NODE_66 | 315 | 41.270 | 684.80 | 710.00 | 622.00 | 335.00 | 0.954 | 0.927 | 0.871 |
NODE_67 | 315 | 36.508 | 1.90 | 2.00 | 1.00 | 1.00 | 0.897 | 0.879 | 0.828 |
NODE_68 | 313 | 40.575 | 2784.46 | 2816.00 | 1334.00 | 633.00 | 0.954 | 0.929 | 0.875 |
NODE_69 | 311 | 42.444 | 491.38 | 423.00 | 94.00 | 5.00 | 0.968 | 0.931 | 0.865 |
NODE_70 | 307 | 41.694 | 14326.13 | 14060.00 | 5774.00 | 2260.00 | 0.959 | 0.919 | 0.847 |
NODE_71 | 306 | 50.327 | 19.62 | 17.00 | 3.00 | 2.00 | 0.982 | 0.963 | 0.901 |
NODE_72 | 305 | 44.262 | 7.70 | 7.00 | 1.00 | 1.00 | 0.972 | 0.954 | 0.898 |
NODE_73 | 304 | 46.711 | 10.71 | 7.00 | 1.00 | 0.00 | 0.970 | 0.942 | 0.883 |
NODE_74 | 301 | 38.870 | 7.26 | 8.00 | 7.00 | 2.00 | 0.959 | 0.931 | 0.867 |
NODE_75 | 298 | 33.893 | 25.91 | 18.00 | 13.00 | 5.00 | 0.936 | 0.914 | 0.855 |
NODE_76 | 298 | 35.235 | 9.32 | 6.00 | 2.00 | 1.00 | 0.960 | 0.926 | 0.865 |
NODE_77 | 297 | 49.158 | 1.02 | 1.00 | 1.00 | 1.00 | 0.971 | 0.952 | 0.887 |
NODE_78 | 294 | 55.442 | 1.52 | 1.00 | 1.00 | 1.00 | 0.985 | 0.968 | 0.909 |
NODE_79 | 294 | 46.939 | 1.54 | 2.00 | 1.00 | 1.00 | 0.977 | 0.950 | 0.885 |
NODE_80 | 293 | 43.345 | 7897.85 | 8942.00 | 3297.00 | 1413.00 | 0.967 | 0.926 | 0.859 |
NODE_81 | 293 | 49.829 | 14.19 | 10.00 | 7.00 | 4.00 | 0.986 | 0.971 | 0.907 |
NODE_82 | 293 | 46.758 | 8.60 | 3.00 | 1.00 | 0.00 | 0.983 | 0.966 | 0.903 |
NODE_83 | 292 | 45.548 | 2.07 | 2.00 | 2.00 | 2.00 | 0.988 | 0.967 | 0.895 |
NODE_84 | 292 | 46.918 | 3.16 | 4.00 | 1.00 | 1.00 | 0.980 | 0.956 | 0.885 |
NODE_85 | 291 | 34.021 | 3.71 | 4.00 | 1.00 | 0.00 | 0.950 | 0.901 | 0.837 |
NODE_86 | 290 | 41.379 | 6.68 | 5.00 | 2.00 | 1.00 | 0.955 | 0.913 | 0.851 |
NODE_87 | 290 | 41.724 | 66.89 | 50.00 | 14.00 | 10.00 | 0.960 | 0.928 | 0.864 |
NODE_88 | 287 | 44.251 | 8.97 | 11.00 | 3.00 | 1.00 | 0.966 | 0.931 | 0.864 |
NODE_89 | 287 | 41.812 | 78.16 | 14.00 | 12.00 | 7.00 | 0.972 | 0.941 | 0.883 |
NODE_90 | 287 | 31.707 | 2.38 | 2.00 | 2.00 | 2.00 | 0.888 | 0.868 | 0.816 |
NODE_91 | 287 | 37.282 | 3.01 | 3.00 | 2.00 | 1.00 | 0.924 | 0.904 | 0.853 |
NODE_92 | 286 | 39.510 | 2140.09 | 2266.00 | 1187.00 | 710.00 | 0.964 | 0.939 | 0.866 |
NODE_93 | 286 | 44.406 | 36.46 | 38.00 | 14.00 | 8.00 | 0.970 | 0.927 | 0.860 |
NODE_94 | 283 | 51.943 | 2.13 | 2.00 | 2.00 | 2.00 | 0.955 | 0.921 | 0.855 |
NODE_95 | 281 | 56.228 | 19.95 | 23.00 | 11.00 | 1.00 | 0.985 | 0.963 | 0.903 |
NODE_96 | 281 | 31.317 | 9.78 | 8.00 | 7.00 | 5.00 | 0.919 | 0.896 | 0.841 |
NODE_97 | 281 | 30.961 | 2.14 | 2.00 | 1.00 | 1.00 | 0.866 | 0.796 | 0.728 |
NODE_98 | 281 | 43.060 | 29.21 | 26.00 | 16.00 | 8.00 | 0.961 | 0.928 | 0.860 |
NODE_99 | 280 | 45.000 | 1.07 | 1.00 | 1.00 | 1.00 | 0.976 | 0.955 | 0.885 |
NODE_100 | 271 | 44.649 | 10070.13 | 10850.00 | 5719.00 | 2844.00 | 0.962 | 0.922 | 0.855 |
NODE_101 | 267 | 42.697 | 3375.30 | 3476.00 | 1820.00 | 872.00 | 0.938 | 0.912 | 0.850 |
NODE_102 | 255 | 40.784 | 2399.00 | 2405.00 | 1543.00 | 776.00 | 0.950 | 0.916 | 0.853 |
NODE_103 | 223 | 43.498 | 17720.84 | 19196.00 | 8262.00 | 4395.00 | 0.959 | 0.915 | 0.841 |
NODE_104 | 216 | 41.667 | 1312.00 | 1374.00 | 761.00 | 423.00 | 0.940 | 0.899 | 0.829 |
NODE_105 | 211 | 41.232 | 1142.41 | 1290.00 | 631.00 | 313.00 | 0.954 | 0.916 | 0.846 |
NODE_106 | 197 | 42.640 | 15573.62 | 16793.00 | 8736.00 | 4358.00 | 0.966 | 0.919 | 0.835 |
NODE_107 | 192 | 44.271 | 17683.44 | 20988.00 | 11641.00 | 6096.00 | 0.951 | 0.905 | 0.826 |
NODE_108 | 185 | 42.162 | 1536.76 | 1850.00 | 989.00 | 411.00 | 0.958 | 0.922 | 0.836 |
NODE_109 | 171 | 40.351 | 275.58 | 315.00 | 169.00 | 66.00 | 0.925 | 0.869 | 0.790 |
NODE_110 | 166 | 43.373 | 2126.71 | 2422.00 | 1972.00 | 1003.00 | 0.967 | 0.913 | 0.817 |
NODE_111 | 142 | 40.845 | 105.83 | 113.00 | 93.00 | 72.00 | 0.966 | 0.879 | 0.782 |
NODE_112 | 128 | 100.000 | 3773.05 | 3725.00 | 3472.00 | 3061.00 | 0.000 | 0.000 | 0.000 |
NODE_113 | 128 | 30.469 | 19.09 | 20.00 | 18.00 | 16.00 | 0.920 | 0.870 | 0.781 |
Details
- Method: Bakta
-
Rapid & standardized annotation of bacterial genomes, MAGs & plasmids. - --force
Table 1. Annotation details Download table data
Sample | tRNAs | tmRNAs | rRNAs | ncRNAs | ncRNA regions | CRISPR arrays | CDSs | pseudogenes | hypotheticals | signal peptides | sORFs | gaps | oriCs | oriVs | oriTs | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
example | 52 | 1 | 6 | 10 | 24 | 0 | 1720 | 20 | 102 | 0 | 4 | 0 | 3 | 0 | 1 |