Sample Alignments, duplicates removed Alignments PCR duplicates Unaligned reads Percent aligned Percent non-duplicates RNA_mouse_polyA_folB.1 9251608 21588988 12337380 593979 97.3 % 41.7 % RNA_mouse_polyA_folB.2 9663713 22224300 12560587 620556 97.3 % 42.3 % RNA_mouse_polyA_pre-proB.1 9709200 22608292 12899092 660158 97.2 % 41.7 % RNA_mouse_polyA_pre-proB.2 9442179 21741704 12299525 610499 97.3 % 42.2 %